The Real "Age-Killing-Effect" SYN@-AKE: synthetic substance mimics snake venom activity. Snake venom can be deadly - but similar substances can also heal!! The form. Of a "wrinkle-killer" it is being used to help boost the self-confidence of both men and women. SYN@-AKE is a synthetic substance which mimics part of a polypeptide found in the venom of the Temple Viper. It has beneficial effects for the skin - perhaps "Love at first bite”.

Lines caused by facial expressions are always visible. We use about 60 facial muscles whilst communicating both through speech and with facial expressions. Frowning, the lifting of eyebrows. Laughing and pulling a face are some typical facial expressions that we use to communicate. As time passes and with frequent use of these facial movements, these wrinkles persist and become permanent deep wrinkles on the forehead, laughter lines and crow's feet. Those who no longer want to live with these can now spare themselves the danger and discomfort of cosmetic surgery or the use of expensive injections.

SYN@-AKE solves this problem on a strictly cosmetic basis. The new synthetic Tripeptide mimics the effect of Waglerin-1. Which is a protein found in the venom of the Temple Viper (Tropidolaemus wagleri). The viper owes its name to the German herpe- tologist Johann Georg Wagler. At the beginning of the 19th century, he ana- lysed and described it. The Templ4~ Viper is found in great numbers in the Snake Temple of the island Penang. Malaysia. It has enjoyed cult status there for the last 150 years. Waglerin-'! Inhibits neuromuscular communication. Once the snake has bitten its prey. the venom blocks the nerve impulses to the muscles, thereby paralysing its victim. Whereas this causes death, the synthetic imitation of parts of the venom only causes relaxation of facial muscle contractions, resulting in a smooth skin and a renewed enjoyment of life.

The effectiveness of this substance is astounding: it has excellent skin- smoothing properties and reduces the depth of facial wrinkles in just a few days. A study involving 45 volunteers confirmed these results. During this test, the use of SYN@-AKE twice daily-reduced forehead wrinkles, by up to 52% in just 28 days. The in vivo studies dealing with crow's feet con- firmed the efficiency of the product. In vitro studies show that SYN@-AKE, in a concentration of 0.5 mm, reduces the frequency of muscle contractions in innervated muscle cells by 82% (p<O.05) within only 2 hours.

The active compound SYN@-AKE is the result of Peptides synthesis and expertise in snake venom research. The researchers at Pentapharm have managed to make a remarkable transference from the effects of snake venom peptides, which consist of 22 amino acids to a peptide-like com- pound containing only 3 amino acids. This kind of research has long been a tradition at Pentapharm. For 30 years, snake venom has been used for therapeutic and diagnostic purposes. One example is the utilization of the anti-coagulant properties of parts of the South American Lance Adder venom. To this purpose, Pentapharm in Brazil maintains a snake farm, housing and breeding about 10,000 snakes.

The venom is milked locally in Brazil. Then transported to Switzerland for purification and further processing.

The synthetic peptide SYN@-AKE thus enables Pentapharm to dispense with the use of the snake farm. SYN@-AKE is safe. Indisputably, ecology-friendly and totally without risk, during production (in line with our "Responsible Care Program"). It contains no preservative and is considered absolutely safe for use in cosmetic products.


Abstract
New Age-Killing Effect

Pentapharm Ltd. introduces its new anti- wrinkle active compound based on a synthetic tripeptide that mimics the effect of Waglerin-1, a peptide that is found in the venom of the Temple Viper (Tropi- dolaemus wagleri). Pentapharm has developed. During the past 30 Years, a unique approach for the breeding and housing of venomous snakes and particularly the Brazilian Lance Adder (Bothrops moojeni), whose venom is used for therapeutic (anticoagulants, haemostatics) and diagnostic products. The long experience of Pentapharm in snake venom research has made possible the investigation of venom Peptides for cosmetic applications. The first focus has been the investigation of the Temple Viper's venom.

Waglerin-1, a protein contained in the venom of the snake Tropidotaemus wagleri, has the property of blocking neuromuscular contraction. Following extensive wide screening, the development of a synthetic peptide tl1at mimics the activity of the selected protein has been made possible. SYN@-AKE is offered as a glycerine- based aqueous solution. In vitro tests have shown that SYN@-AKE significantly reduces the frequency of con- traction of innervated muscle cells as a function of the incubation time. In vivo tests show the excellent anti-wrinkle activity. SYN@-AKE has been thoroughly tested and is considered to be appropriate and safe for cosmetic application.